PIoreactor vial cap for electrolysis and CO2 sparging

This cap has six holes so that you can insert electrodes for in-situ electrolysis and lines for CO2 sparging, in addition to the inflow/outflow lines.

There are two different designs with the holes in different orientations.

A special thank you to Rob Jess for creating this design!

Printing

Important: only use high-temperature plastics if you plan to heat-sterilize this cap. We use Prusa's Polycarbonate and Polycarbonate-CF

• 3 vertical shell perimeters
• 0.15mm layer height
• Print with the top-side down for best results

Adding an o-ring insert underneath

To avoid leaks, we add a 1/16" W, 3/4" ID, 7/8" OD o-ring underneath. It's secured with some high-temperature silicone. Tip: we put the uncured silicone into a syringe to apply. 

This design add extra holes in different orientations to the Pioreactor vial cap, so that you can insert electrodes for in-situ electrolysis and lines for CO2 sparging, in addition to the inflow/outflow lines.