This calibration takes into account all your printer profile's first layer settings, giving a perfect Z-Offset. Use an opaque filament.
If you are really anal like me, you can use a set of calipers to make sure the print is 0.25 mm. If you get to 0.25 mm and the lines aren't joined, then increase your first layer flow. Repeat until the lines of the print are joined and smooth.
Good Luck, and Happy Printing!
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