Screw Top Flow Cell

Grace bio-labs has really handy perfusion cells for cell biology studies. They consist of a thin plastic sheet with an inlet and outlet hole, and an adhered silicone gasket. These stick electrostatically to coverslips to create a microscopy perfusion chamber. In our work, we need to use these under flow, which can result in leakage under certain pressures/flow rates. To use these in a flow application, they can be mounted in this rig, which uniformly applies pressure to the gasket, eliminating leakage in tests up to 100 mbar applied pressure. 

As shown, this is printed at 0.1 mm layer height without supports in PLA. It is important to resize the .stl for the bottom piece to 99% in the X and Y dimensions for smooth threading. This is already done in the slicer file uploaded here.